Identification and validation of a major quantitative trait locus for spike length and compactness in the wheat (Triticum aestivum L.) line Chuanyu12D7

Spike length (SL) and spike compactness (SC) are crucial traits related to wheat ( Triticum aestivum L.) yield potential. In this study, a backcrossed inbred lines (BILs) population segregating for SL/SC was developed by using commercial variety chuanyu25 as recurrent parent backbone Chuanyu12D7. Bulked segregant analysis (BSA) combined with the Wheat 660K SNP array performed conduct quantitative trait locus (QTL) mapping. A major stable QTL (designated QSl/Sc.cib-2D.1 ) identified on chromosome 2DS, explaining 45.63-59.72% of phenotypic variation. mapped 102.29-Kb interval flanking SNPs AX-110276364 AX-111593853 BC 4 F 2:3 population. Since is linked Rht8 gene, their additive effects plant type were analysed. Remarkably, superior allele can increase SL TGW, decrese SC without any apparent trade-offs in other yield-related traits. addition, closely kompetitive allele-specific PCR (KASP) markers marker-assisted selection (MAS) breeding. Four genes within physical considered potential candidates based expression patterns well orthologous gene functions. These results laid foundation map-based cloning gene(s) underlying its application ideotype